Transcription, Translation, and Cellular Localization of PDV-E66: A Structural Protein of the PDV Envelope of Autographa californica Nuclear Polyhedrosis Virus
Identifieur interne : 004373 ( Main/Exploration ); précédent : 004372; suivant : 004374Transcription, Translation, and Cellular Localization of PDV-E66: A Structural Protein of the PDV Envelope of Autographa californica Nuclear Polyhedrosis Virus
Auteurs : Tao Hong [États-Unis] ; Sharon C. Braunagel [États-Unis] ; Max D. Summers [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1994.
Abstract
Abstract: A late gene encoding a 66-kDa structural protein of Autographa californica nuclear polyhedrosis virus was mapped and sequenced (26.9-297 MU). Transcription initiates from two conserved TAAG motifs (-15 and -37) with transcripts detected from 12 to 72 hr pi. The protein is detected in infected Spodoptera frugiperda (Sf9) cells from 24 to 72 hr pi. Western blot and immunoelectron microscopic data identify this protein as specific for the polyhedra-derived virus (PDV) envelope. This protein has been named PDV-E66 to identify its viral origin, envelope location, and apparent molecular weight. In addition to being a structural protein of the PDV envelope, PDV-E66 is enriched in loci of microvesicles in the nuclei of infected Sf9 cells.
Url:
DOI: 10.1006/viro.1994.1525
Affiliations:
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Braunagel</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>Departments of Biology, Entomology, Biochemistry and Biophysics, Texas Agriculture Experiment Station, and Center for Advanced Invertebrate Molecular Sciences, Institute of Biosciences and Technology, Texas A & M University, College Station</wicri:cityArea></affiliation></author><author><name sortKey="Summers, Max D" sort="Summers, Max D" uniqKey="Summers M" first="Max D." last="Summers">Max D. Summers</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>Departments of Biology, Entomology, Biochemistry and Biophysics, Texas Agriculture Experiment Station, and Center for Advanced Invertebrate Molecular Sciences, Institute of Biosciences and Technology, Texas A & M University, College Station</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">204</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="210">210</biblScope><biblScope unit="page" to="222">222</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A late gene encoding a 66-kDa structural protein of Autographa californica nuclear polyhedrosis virus was mapped and sequenced (26.9-297 MU). Transcription initiates from two conserved TAAG motifs (-15 and -37) with transcripts detected from 12 to 72 hr pi. The protein is detected in infected Spodoptera frugiperda (Sf9) cells from 24 to 72 hr pi. Western blot and immunoelectron microscopic data identify this protein as specific for the polyhedra-derived virus (PDV) envelope. This protein has been named PDV-E66 to identify its viral origin, envelope location, and apparent molecular weight. In addition to being a structural protein of the PDV envelope, PDV-E66 is enriched in loci of microvesicles in the nuclei of infected Sf9 cells.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><country name="États-Unis"><region name="Texas"><name sortKey="Hong, Tao" sort="Hong, Tao" uniqKey="Hong T" first="Tao" last="Hong">Tao Hong</name></region><name sortKey="Braunagel, Sharon C" sort="Braunagel, Sharon C" uniqKey="Braunagel S" first="Sharon C." last="Braunagel">Sharon C. Braunagel</name><name sortKey="Summers, Max D" sort="Summers, Max D" uniqKey="Summers M" first="Max D." last="Summers">Max D. Summers</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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